polyclonal goat anti ephrinb2 Search Results


polyclonal goat anti ephrinb2  (R&D Systems)
90
R&D Systems polyclonal goat anti ephrinb2
Polyclonal Goat Anti Ephrinb2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal goat anti ephrinb2/product/R&D Systems
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recombinant human egf protein, cf  (Bio-Techne corporation)
99
Bio-Techne corporation recombinant human egf protein, cf
Recombinant Human Egf Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human egf protein, cf/product/Bio-Techne corporation
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goat anti ephrinb2  (R&D Systems)
93
R&D Systems goat anti ephrinb2
Goat Anti Ephrinb2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti ephrinb2/product/R&D Systems
Average 93 stars, based on 1 article reviews
goat anti ephrinb2 - by Bioz Stars, 2026-03
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polyclonal anti ephrinb2 goat antibodies  (R&D Systems)
93
R&D Systems polyclonal anti ephrinb2 goat antibodies
Polyclonal Anti Ephrinb2 Goat Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal anti ephrinb2 goat antibodies/product/R&D Systems
Average 93 stars, based on 1 article reviews
polyclonal anti ephrinb2 goat antibodies - by Bioz Stars, 2026-03
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goat anti mouse ephrinb2  (R&D Systems)
90
R&D Systems goat anti mouse ephrinb2
Goat Anti Mouse Ephrinb2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse ephrinb2/product/R&D Systems
Average 90 stars, based on 1 article reviews
goat anti mouse ephrinb2 - by Bioz Stars, 2026-03
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pla probes  (Thermo Fisher)
90
Thermo Fisher pla probes
<t>EphrinB2</t> associates with <t>SHP2,</t> <t>JAK2</t> and <t>STAT1,</t> and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated <t>with</t> <t>antibodies</t> to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) <t>PLA</t> shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Pla Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pla probes/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
pla probes - by Bioz Stars, 2026-03
90/100 stars
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goat anti ephrinb2  (Neuromics)
91
Neuromics goat anti ephrinb2
<t>EphrinB2</t> associates with <t>SHP2,</t> <t>JAK2</t> and <t>STAT1,</t> and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated <t>with</t> <t>antibodies</t> to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) <t>PLA</t> shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Goat Anti Ephrinb2, supplied by Neuromics, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti ephrinb2/product/Neuromics
Average 91 stars, based on 1 article reviews
goat anti ephrinb2 - by Bioz Stars, 2026-03
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biotin anti ephrinb2  (R&D Systems)
91
R&D Systems biotin anti ephrinb2
<t>EphrinB2</t> associates with <t>SHP2,</t> <t>JAK2</t> and <t>STAT1,</t> and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated <t>with</t> <t>antibodies</t> to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) <t>PLA</t> shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Biotin Anti Ephrinb2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin anti ephrinb2/product/R&D Systems
Average 91 stars, based on 1 article reviews
biotin anti ephrinb2 - by Bioz Stars, 2026-03
91/100 stars
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antibody anti human mouse rat ephrinb2  (R&D Systems)
90
R&D Systems antibody anti human mouse rat ephrinb2
<t>EphrinB2</t> associates with <t>SHP2,</t> <t>JAK2</t> and <t>STAT1,</t> and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated <t>with</t> <t>antibodies</t> to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) <t>PLA</t> shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
Antibody Anti Human Mouse Rat Ephrinb2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti human mouse rat ephrinb2/product/R&D Systems
Average 90 stars, based on 1 article reviews
antibody anti human mouse rat ephrinb2 - by Bioz Stars, 2026-03
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50 g/ml goat anti-mouse ephrinb2  (Agilent technologies)
90
Agilent technologies 50 g/ml goat anti-mouse ephrinb2
<t>EphrinB2</t> associates with <t>SHP2,</t> <t>JAK2</t> and <t>STAT1,</t> and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated <t>with</t> <t>antibodies</t> to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) <t>PLA</t> shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.
50 G/Ml Goat Anti Mouse Ephrinb2, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/50 g/ml goat anti-mouse ephrinb2/product/Agilent technologies
Average 90 stars, based on 1 article reviews
50 g/ml goat anti-mouse ephrinb2 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


EphrinB2 associates with SHP2, JAK2 and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.

Journal: Nature communications

Article Title: EphrinB2 controls vessel pruning through STAT1-JNK3 signaling

doi: 10.1038/ncomms7576

Figure Lengend Snippet: EphrinB2 associates with SHP2, JAK2 and STAT1, and modulates STAT1 activity. (a,b) HUVEC infected with eB2-shRNA or eB2-5Y express higher levels of p-STAT1 compared to controls; p-STAT3 levels are similar. Flow cytometry (a; % positive cells is noted on each quadrant; representative of 5 experiments), immunoblotting (b);. (c) Nuclear localization of p-STAT1 (red, arrowheads) in HUVEC transduced with eB2-5Y (GFP: green); little p-STAT1 (red) is detected in HUVEC transduced with eB2-WT (GFP: green); representative images. Scale bar: 20μm (d) EphB4-Fc activates endogenous p-EphrinB and reduces p-STAT1 levels; quantitation (average fluorescence intensity/cell). Results reflect the means±SD from 3 experiments; P values from two-tailed Student t -test: N.S., non significant, * P <0.05, ** P <0.01; error bars: ±SD. (e) Serum starvation time-dependently reduces p-EphrinB levels and increases p-STAT1 levels in HUVEC. (f) EphrinB2 associates with SHP2, JAK2 and STAT1 in HUVEC; cell lysates of HUVEC were immunoprecipitated with antibodies to EphrinB2 or control IgG; precipitates and cell lysates were immunoblotted as indicated. (g–i) Quantitative analysis of EphrinB2 association with SHP2, JAK2 and STAT1 in p5 hyaloid vessels from EphrinB2 WT/WT (n=5) and EphrinB2 5Y/5Y (n=7) mice detected by PEA (top panels). Negative control: reagents alone, no cell lysate. SHP2, JAK2 and STAT1 abundance in PEA input samples (bottom). (j) PLA shows that p-EphrinB associates with SHP2 in hyaloid vessels from EphrinB2 WT/WT mice but not from EphrinB2 5Y/5Y mice. Red: EphrinB2+SHP2; blue: DAPI. Dotted line limits amplified areas in lower panels. Scale bars: 500μm (top panels), 100μm (bottom panels) (k) Quantitation of p-EphrinB+SHP2 proximity co-localization in WT hyaloid vessels regions at p5 and p7. Results (mean±SD fluorescence intensity/mm 2 area) are normalized with DAPI. Error bars: ±SD.

Article Snippet: After washing, cells, hyaloid vessels and sections were incubated with secondary antibodies with PLA probes (MINUS probe-conjugated anti-rabbit IgG+PLUS probe-conjugated anti-mouse IgG for JNK3, EphrinB2+STAT1 and EphrinB2+-STAT1 detection; PLUS probe-conjugated anti-goat IgG+MINUS probe-conjugated anti-rabbit IgG for detection of EphrinB2+JAK2, EphrinB2+STAT1, and EphrinB2+SHP2; Olink Bioscience, Uppsala, Sweden).

Techniques: Activity Assay, Infection, shRNA, Flow Cytometry, Western Blot, Transduction, Quantitation Assay, Fluorescence, Two Tailed Test, Immunoprecipitation, Control, Negative Control, Amplification

Sustained EphrinB2 phosphorylation in endothelial cells within the retrolental mass of PHPV. (a) Retrolental mass and retinal degeneration in PHPV. Cross section of PHPV eyeball stained with H&E (representative of 6 samples). Center panel: tiled image of the entire eye section showing the characteristic retrolental mass and retinal detachment; scale bar: 5mm. Left panel: magnified image showing retinal degeneration; scale bar: 100μm. Righteft panel: magnified image showing fibrovascular tissue within the retrolental mass; scale bar: 100μm. (b) Endothelial cells (CD31 + ) within the PHPV retrolental mass are p-EphrinB + and JNK3 − . Staining with anti-EphrinB (green), anti-JNK3 (white), anti-CD31 (red) and DAPI (blue). Arrowheads point to CD31 + p-EphrinB + JNK3 − cells. Scale bars: 1mm (left panel), 100μm (right panels) (c) Proximity co-localization of p-EphrinB and SHP2 in endothelial cells in PHPV retrolental mass. Red: PLA signal from p-EphrinB/SHP2 in section of PHPV retrolental mass; green: CD31 immunostaining; blue: DAPI. V: vessel. Arrowheads point to p-EphrinB + SHP2 + cells. Scale bars: 10μm. (d) PLA showing co-localization of EphrinB2 and STAT1 in endothelial cells from PHPV retrolental mass. Red: PLA signal from EphrinB2/STAT1, green: CD31; blue: DAPI. Asterisk: red blood cell. Arrowheads point to EphrinB2 + STAT1 + cells. Scale bars: 50μm. (e) Co-localization of EphrinB2 and p-STAT1 is limited in endothelial cells in PHPV retrolental mass. Red: PLA signal from EphrinB2/p-STAT1, green: CD31; blue: DAPI. Asterisk: red blood cell. Arrows point toEphrinB2 + p-STAT1 + cells. Scale bars: 50μm.

Journal: Nature communications

Article Title: EphrinB2 controls vessel pruning through STAT1-JNK3 signaling

doi: 10.1038/ncomms7576

Figure Lengend Snippet: Sustained EphrinB2 phosphorylation in endothelial cells within the retrolental mass of PHPV. (a) Retrolental mass and retinal degeneration in PHPV. Cross section of PHPV eyeball stained with H&E (representative of 6 samples). Center panel: tiled image of the entire eye section showing the characteristic retrolental mass and retinal detachment; scale bar: 5mm. Left panel: magnified image showing retinal degeneration; scale bar: 100μm. Righteft panel: magnified image showing fibrovascular tissue within the retrolental mass; scale bar: 100μm. (b) Endothelial cells (CD31 + ) within the PHPV retrolental mass are p-EphrinB + and JNK3 − . Staining with anti-EphrinB (green), anti-JNK3 (white), anti-CD31 (red) and DAPI (blue). Arrowheads point to CD31 + p-EphrinB + JNK3 − cells. Scale bars: 1mm (left panel), 100μm (right panels) (c) Proximity co-localization of p-EphrinB and SHP2 in endothelial cells in PHPV retrolental mass. Red: PLA signal from p-EphrinB/SHP2 in section of PHPV retrolental mass; green: CD31 immunostaining; blue: DAPI. V: vessel. Arrowheads point to p-EphrinB + SHP2 + cells. Scale bars: 10μm. (d) PLA showing co-localization of EphrinB2 and STAT1 in endothelial cells from PHPV retrolental mass. Red: PLA signal from EphrinB2/STAT1, green: CD31; blue: DAPI. Asterisk: red blood cell. Arrowheads point to EphrinB2 + STAT1 + cells. Scale bars: 50μm. (e) Co-localization of EphrinB2 and p-STAT1 is limited in endothelial cells in PHPV retrolental mass. Red: PLA signal from EphrinB2/p-STAT1, green: CD31; blue: DAPI. Asterisk: red blood cell. Arrows point toEphrinB2 + p-STAT1 + cells. Scale bars: 50μm.

Article Snippet: After washing, cells, hyaloid vessels and sections were incubated with secondary antibodies with PLA probes (MINUS probe-conjugated anti-rabbit IgG+PLUS probe-conjugated anti-mouse IgG for JNK3, EphrinB2+STAT1 and EphrinB2+-STAT1 detection; PLUS probe-conjugated anti-goat IgG+MINUS probe-conjugated anti-rabbit IgG for detection of EphrinB2+JAK2, EphrinB2+STAT1, and EphrinB2+SHP2; Olink Bioscience, Uppsala, Sweden).

Techniques: Phospho-proteomics, Staining, Immunostaining